Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
FITC,
HRP,
Biotin.
Purification
Purified IgG
Immunogen
Highly purified monoclonal IgE isolated from human serum.
Specificity
Human IgE, isotype specific.
Usage
The reactivity of the antiserum is restricted to the IgE molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. in ELISA from 1:50 upwards. in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee