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| Catalog Number | Size | Price |
|---|---|---|
| LS-C8914-100 | 100 µg (2 mg/ml) | $842 |
Monoclonal Mouse anti‑Human MMP14 Antibody (aa319‑333, IHC, WB) LS‑C8914
Monoclonal Mouse anti‑Human MMP14 Antibody (aa319‑333, IHC, WB) LS‑C8914
Antibody:
MMP14 Mouse anti-Human Monoclonal (aa319-333) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Hamster, Sheep
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
MMP14 Mouse anti-Human Monoclonal (aa319-333) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Hamster, Sheep
Format:
Unconjugated, Unmodified
Specifications
Description
MMP14 antibody LS-C8914 is an unconjugated mouse monoclonal antibody to MMP14 (aa319-333) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Target
Human MMP14
Synonyms
MMP14 | Membrane type-1 MMP | MT1-MMP | MT1MMP | MT-MMP | MT-MMP 1 | Matrix metalloproteinase-14 | MMP-14 | MMP-X1 | MTMMP1
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Goat, Hamster, Sheep
(tested or 100% immunogen sequence identity)
Clonality
IgG3,k
Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%); Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%); Zebrafish, Xenopus, Medaka (87%); Lizard (80%).
Epitope
aa319-333
Specificity
Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Western blot (10 µg/ml)
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 ug/ml, membrane preparations; MD-MB-231 cells pretreated with 1-5 ug/ml ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections; traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.
Presentation
PBS, pH 7.0, 2% BSA (protease free).
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MMP14
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
