Target
Human MIF
Synonyms
MIF | GLIF | L-dopachrome tautomerase | L-dopachrome isomerase | MMIF | Phenylpyruvate tautomerase
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Recombinant human MIF expressed in E. coli.
Specificity
Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Does not cross-react with other cytokines. Species cross-reactivity: mouse.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Flow Cytometry (3 - 10 µg/ml)
Usage
Suitable for use in ELISA, Flow Cytometry and Western Blot. Flow Cytometry: 3-10 ug/ml labels/1E6 cells. Western Blot: 0.1-0.2 ug/ml. Detection limit for is ~5 ng/lane under non-reducing and reducing conditions.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute with 500uL of sterile PBS.
Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee