Target
Human MIF
Synonyms
MIF | GLIF | L-dopachrome tautomerase | L-dopachrome isomerase | MMIF | Phenylpyruvate tautomerase
Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Immunoaffinity purified
Immunogen
Human MIF, recombinant protein expressed in E. coli.
Specificity
Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Does not cross-react with other cytokines.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Flow Cytometry
- ELISA
Usage
Flow Cytometry: 2.5ug/ml labels 10e6 cells. HL-60 human acute promyelocytic leukemia cell line treated with LPS, fixed with paraformaldehyde and permeabilized with saponin.Western Blot: 0.1ug/ml. Recombinant human MIF. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.4, BSA
Reconstitution
Reconstitute with 250uL sterile PBS.
Storage
Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee