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Antibodies > MCM7 > LS-C357428
 
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Catalog Number Size Price
LS-C357428-10 10 µg $318 
LS-C357428-100 100 µg (0.5 mg/ml) $470 
MCM7 Antibody - MCM7 antibody IHC-paraffin: Human Lung Cancer Tissue.
MCM7 Antibody - MCM7 antibody IHC-paraffin: Rat Intestine Tissue.
MCM7 Antibody - MCM7 antibody IHC-paraffin: Mouse Testis Tissue.
MCM7 Antibody - MCM7 antibody ICC: A549 Cell.
MCM7 Antibody - IF analysis of MCM7 using anti-MCM7 antibody MCM7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MCM7 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MCM7 Antibody - IF analysis of MCM7 using anti-MCM7 antibody MCM7 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MCM7 Antibody overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MCM7 Antibody - IF analysis of MCM7 using anti-MCM7 antibody MCM7 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MCM7 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MCM7 Antibody - MCM7 antibody Western blot. All lanes: Anti MCM7 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: JURKAT Whole Cell Lysate at 40 ug. Lane 6: 22RV1 Whole Cell Lysate at 40 ug. Lane 7: COLO320 Whole Cell Lysate at 40 ug. Lane 8: PC-12 Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.
MCM7 Antibody - MCM7 antibody Western blot. All lanes: Anti MCM7 at 0.5 ug/ml. WB: Recombinant Human MCM7 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
MCM7 Antibody - Flow Cytometry analysis of A431 cells using anti-MCM7 antibody. Overlay histogram showing A431 cells stained with anti-MCM7 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM7 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MCM7 Antibody - Flow Cytometry analysis of U937 cells using anti-MCM7 antibody. Overlay histogram showing U937 cells stained with anti-MCM7 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM7 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MCM7 Antibody - MCM7 antibody IHC-paraffin: Human Lung Cancer Tissue.
MCM7 Antibody - MCM7 antibody IHC-paraffin: Rat Intestine Tissue.
MCM7 Antibody - MCM7 antibody IHC-paraffin: Mouse Testis Tissue.
MCM7 Antibody - MCM7 antibody ICC: A549 Cell.
MCM7 Antibody - IF analysis of MCM7 using anti-MCM7 antibody MCM7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MCM7 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MCM7 Antibody - IF analysis of MCM7 using anti-MCM7 antibody MCM7 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MCM7 Antibody overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MCM7 Antibody - IF analysis of MCM7 using anti-MCM7 antibody MCM7 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MCM7 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MCM7 Antibody - MCM7 antibody Western blot. All lanes: Anti MCM7 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: JURKAT Whole Cell Lysate at 40 ug. Lane 6: 22RV1 Whole Cell Lysate at 40 ug. Lane 7: COLO320 Whole Cell Lysate at 40 ug. Lane 8: PC-12 Whole Cell Lysate at 40 ug. Predicted band size: 81 kD. Observed band size: 81 kD.
MCM7 Antibody - MCM7 antibody Western blot. All lanes: Anti MCM7 at 0.5 ug/ml. WB: Recombinant Human MCM7 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
MCM7 Antibody - Flow Cytometry analysis of A431 cells using anti-MCM7 antibody. Overlay histogram showing A431 cells stained with anti-MCM7 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM7 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MCM7 Antibody - Flow Cytometry analysis of U937 cells using anti-MCM7 antibody. Overlay histogram showing U937 cells stained with anti-MCM7 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM7 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human MCM7 Antibody (aa526‑719, IHC, WB) LS‑C357428

Polyclonal Rabbit anti‑Human MCM7 Antibody (aa526‑719, IHC, WB) LS‑C357428

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C388824
Antibody:
MCM7 Rabbit anti-Human Polyclonal (aa526-719) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357428-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MCM7 Rabbit anti-Human Polyclonal (aa526-719) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MCM7 antibody LS-C357428 is an unconjugated rabbit polyclonal antibody to MCM7 (aa526-719) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human MCM7
Synonyms
MCM7 | CDC47 homolog | CDC47 | Homolog of S. cerevisiae Cdc47 | p1.1-MCM3 | p1CDC47 | p85MCM | PNAS146
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7.
Epitope
aa526-719
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCM7
P33993 NM_005916 NP_005907.3

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Featured Products

MCM7 Antibody - Immunofluorescence analysis of HeLa cells using MCM7 antibody. Blue: DAPI for nuclear staining.
MCM7 Antibody LS‑C331288
Species: Human, Mouse
Applications: Immunofluorescence, Western blot
See all MCM7 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/12/2025
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