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Catalog Number Size Price
LS-C757526-200 200 µl $393 
MAPK3 / ERK1 Antibody - MAPK3/1 Antibody (Center) staining MAPK3/1 in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
MAPK3 / ERK1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling MAPK3/1 with MAPK3/1 Antibody (Center) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
MAPK3 / ERK1 Antibody - All lanes: Anti-MAPK3/1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
MAPK3 / ERK1 Antibody - MAPK3/1 Antibody (Center) staining MAPK3/1 in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
MAPK3 / ERK1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling MAPK3/1 with MAPK3/1 Antibody (Center) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
MAPK3 / ERK1 Antibody - All lanes: Anti-MAPK3/1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Monoclonal Mouse anti‑Human MAPK3 / ERK1 Antibody (clone 1129CT3.4.3, Internal, IHC, IF, WB) LS‑C757526

Monoclonal Mouse anti‑Human MAPK3 / ERK1 Antibody (clone 1129CT3.4.3, Internal, IHC, IF, WB) LS‑C757526

Antibody:
MAPK3 / ERK1 Mouse anti-Human Monoclonal (Internal) (1129CT3.4.3) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757526-200
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MAPK3 / ERK1 Mouse anti-Human Monoclonal (Internal) (1129CT3.4.3) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ERK1 antibody LS-C757526 is an unconjugated mouse monoclonal antibody to ERK1 (MAPK3) (Internal) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Xenopus, Drosophila (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1129CT3.4.3
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
This MAPK3/1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 175-208 amino acids from the Central region of human MAPK3/1.
Epitope
Internal
Applications
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 43.1 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK3 / ERK1
P27361 X60188 CAA42744.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/23/2024