Human MAPK3 / ERK1

Rabbit

Human, Mouse, Rat, Hamster, Chicken (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (KLH).

pThr202/Tyr204

Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). Does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase, or non-phosphorylated Erk1/2. Species cross-reactivity: chicken, hamster, mouse, rat, zebrafish. bovine and monkey.

• ICC
• Immunofluorescence
• Western blot (1:1000)
• Immunoprecipitation (1:50)
• Flow Cytometry (1:100)
• ELISA

Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunofluorescence (IF-IC): 1:1000. Flow Cytometry: 1:100.

10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P27361 NCBI: X60188 CAA42744.1