Target
Human MAPK1 / ERK2
Synonyms
MAPK1 | ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.
Specificity
Detects endogenous levels of p38 MAP kinase only when activated by dual phosphorylation at Thr180 and Tyr182. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182. Does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species cross-reactivity: Human.
Applications
- ICC (1:100)
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- Flow Cytometry (1:100)
- ELISA
Usage
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunofluorescence/ Immunocytochemistry: 1:100. Flow Cytometry: 1:100.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 50% Glycerol, BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee