Target
Human MAPK1 / ERK2
Synonyms
MAPK1 | ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.
Specificity
Detects p38 MAP kinase only when activated by dual phosphorylation at Thr180 and Tyr182. Does not appreciably cross-react with the corresponding phosphorylated forms of either p44/42 MAPK (Erk1/2) or SAPK/JNK. It does not detect non-phosphorylated p38 MAP kinase. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (1:2000)
- Immunoprecipitation (1:50)
- Flow Cytometry (1:1000)
Usage
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Western Blot: 1:2000. Immunoprecipitation: 1:50. Flow Cytometry: 1:1000.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee