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Catalog Number Size Price
LS-C784287-100 100 µg $511 
MAPK1 / ERK2 Antibody - IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAPK1 mouse monoclonal antibody. (Dilution 1:50).
MAPK1 / ERK2 Antibody - IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAPK1 mouse monoclonal antibody. (Dilution 1:50).
MAPK1 / ERK2 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
MAPK1 / ERK2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
MAPK1 / ERK2 Antibody - Immunoprecipitation(IP) of MAPK1 by using monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAPK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
MAPK1 / ERK2 Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK1 antibody, and then analyzed by flow cytometry.
MAPK1 / ERK2 Antibody - IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-MAPK1 mouse monoclonal antibody. (Dilution 1:50).
MAPK1 / ERK2 Antibody - IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAPK1 mouse monoclonal antibody. (Dilution 1:50).
MAPK1 / ERK2 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
MAPK1 / ERK2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
MAPK1 / ERK2 Antibody - Immunoprecipitation(IP) of MAPK1 by using monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAPK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
MAPK1 / ERK2 Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK1 antibody, and then analyzed by flow cytometry.
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Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone OTI6F8, Carrier‑free, IHC, WB) LS‑C784287

Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone OTI6F8, Carrier‑free, IHC, WB) LS‑C784287

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (Carrier-free) (OTI6F8) Antibody
Application:
IHC, WB, IP, Flo
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$511
LS-C784287-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (Carrier-free) (OTI6F8) Antibody
Application:
IHC, WB, IP, Flo
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
ERK2 antibody LS-C784287 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) from human. It is reactive with human, rat, dog and other species. Validated for Flow, IHC, IP and WB.
Target
Human MAPK1 / ERK2
Synonyms
MAPK1 | ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Host
Mouse
Reactivity
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
OTI6F8
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.
Applications
  • IHC (1:50)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (4 µg/ml)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK1 / ERK2
P28482 NM_002745 NP_002736.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/29/2024