Human LRP5

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the C-Terminus of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.

C-Terminus

Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species cross-reactivity: Mouse.

• Immunofluorescence (2 - 3 µg/ml)
• Western blot (1 - 3 µg/ml)

Suitable for use in Immunofluorescence and Western Blot. Immunofluorescence: 2-3 ug/ml; Note: For optimal results in immunofluorescence assays, cells should be fixed in 4% paraformaldehyde/0.5% Triton X-100/PBS for 10 minutes, permeabilized with 0.5% Triton X-100/PBS for 5 minutes, and blocked with 5% BSA/PBS for 30 minutes. Western Blot: 1-3 ug/ml; Single band at ~200kD. Positive control: Mouse LRP5-pSEC-tag-transfected 293T cells, MCF-7 human breast adenocarcinoma, T47D human breast ductal carcinoma, and NIH-3T3 mouse embryo fibroblast lysates.

PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O75197 NCBI: NM_002335 NP_002326.2