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Catalog Number Size Price
LS-C407824-10 10 µg $318 
LS-C407824-100 100 µg $470 
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody Western blot. All lanes: Anti Lamin B1 at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: PC-12 Whole Cell Lysate at 40 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 67 kD. Observed band size: 67 kD.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LMNB1 / Lamin B1 Antibody - Lamin B1 antibody Western blot. All lanes: Anti Lamin B1 at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: PC-12 Whole Cell Lysate at 40 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 67 kD. Observed band size: 67 kD.
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Polyclonal Rabbit anti‑Human LMNB1 / Lamin B1 Antibody (aa266‑583, IHC, WB) LS‑C407824

Polyclonal Rabbit anti‑Human LMNB1 / Lamin B1 Antibody (aa266‑583, IHC, WB) LS‑C407824

Antibody:
LMNB1 / Lamin B1 Rabbit anti-Human Polyclonal (aa266-583) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407824-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LMNB1 / Lamin B1 Rabbit anti-Human Polyclonal (aa266-583) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Lamin B1 antibody LS-C407824 is an unconjugated rabbit polyclonal antibody to Lamin B1 (LMNB1) (aa266-583) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human LMNB1 / Lamin B1
Synonyms
LMNB1 | ADLD | Lamin B1 | Lamin-B1 | LMN | LMNB | LMN2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively.
Epitope
aa266-583
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LMNB1 / Lamin B1
P20700 NM_005573 NP_005564.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024