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Catalog Number	Size	Price
LS-C407824-10	10 µg	$318
LS-C407824-100	100 µg	$470

LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.

LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

LMNB1 / Lamin B1 Antibody - Lamin B1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LMNB1 / Lamin B1 Antibody - IHC analysis of Lamin B1 using anti-Lamin B1 antibody. Lamin B1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lamin B1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LMNB1 / Lamin B1 Antibody - Lamin B1 antibody Western blot. All lanes: Anti Lamin B1 at 0.5 ug/ml. Lane 1: U87 Whole Cell Lysate at 40 ug. Lane 2: PC-12 Whole Cell Lysate at 40 ug. Lane 3: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 67 kD. Observed band size: 67 kD.

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Polyclonal Rabbit anti‑Human LMNB1 / Lamin B1 Antibody (aa266‑583, IHC, WB) LS‑C407824

Name: Anti-LMNB1 / Lamin B1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407824-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

LMNB1 / Lamin B1 Rabbit anti-Human Polyclonal (aa266-583) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C407824-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

LMNB1 / Lamin B1 Rabbit anti-Human Polyclonal (aa266-583) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Lamin B1 antibody LS-C407824 is an unconjugated rabbit polyclonal antibody to Lamin B1 (LMNB1) (aa266-583) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human LMNB1 / Lamin B1

Synonyms

See All LMNB1 / Lamin B1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively.

Epitope

aa266-583

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About LMNB1 / Lamin B1

Uniprot: P20700 NCBI: NM_005573 NP_005564.1