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Catalog Number Size Price
LS-C352456-100 100 µl (1 mg/ml) $299 
LIMK2 Antibody - Immunohistochemical analysis of LIMK2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
LIMK2 Antibody - Immunofluorescent analysis of LIMK2 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
LIMK2 Antibody - Western blot analysis of LIMK2 expression in Jurkat (A); COLO205 (B); MCF7 (C) whole cell lysates.
LIMK2 Antibody - Immunohistochemical analysis of LIMK2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
LIMK2 Antibody - Immunofluorescent analysis of LIMK2 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
LIMK2 Antibody - Western blot analysis of LIMK2 expression in Jurkat (A); COLO205 (B); MCF7 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human LIMK2 Antibody (Internal, IHC, IF, WB) LS‑C352456

Polyclonal Rabbit anti‑Human LIMK2 Antibody (Internal, IHC, IF, WB) LS‑C352456

Antibody:
LIMK2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352456-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LIMK2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LIMK2 antibody LS-C352456 is an unconjugated rabbit polyclonal antibody to human LIMK2 (Internal). Validated for ICC, IF, IHC and WB.
Target
Human LIMK2
Synonyms
LIMK2 | LIM domain kinase 2 | LIM kinase 2 | LIMK-2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human LIMK2.
Epitope
Internal
Specificity
Recognizes endogenous levels of LIMK2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIMK2
P53671 NM_016733 NP_057952.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024