Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C801113-100 100 µl (1 mg/ml) $379 
LS-C801113-200 200 µl (1 mg/ml) $421 
LIMK1 / LIMK Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
LIMK1 / LIMK Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
LIMK1 / LIMK Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
LIMK1 / LIMK Antibody - Western blot analysis of LIMK1/2 phosphorylation expression in HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
LIMK1 / LIMK Antibody - Western blot analysis of Phospho-LIMK1/2 (Thr508/505) expression in various lysates
LIMK1 / LIMK Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
LIMK1 / LIMK Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
LIMK1 / LIMK Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
LIMK1 / LIMK Antibody - Western blot analysis of LIMK1/2 phosphorylation expression in HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
LIMK1 / LIMK Antibody - Western blot analysis of Phospho-LIMK1/2 (Thr508/505) expression in various lysates
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human LIMK1 / LIMK Antibody (phospho‑Thr508,phospho‑Thr505, IHC, IF, WB) LS‑C801113

Polyclonal Rabbit anti‑Human LIMK1 / LIMK Antibody (phospho‑Thr508,phospho‑Thr505, IHC, IF, WB) LS‑C801113

Antibody:
LIMK1 / LIMK Rabbit anti-Human Polyclonal (pThr508,pThr505) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801113-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LIMK1 / LIMK Rabbit anti-Human Polyclonal (pThr508,pThr505) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
LIMK antibody LS-C801113 is an unconjugated rabbit polyclonal antibody to LIMK (LIMK1) (pThr508,pThr505) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human LIMK1 / LIMK
Synonyms
LIMK1 | LIM domain kinase 1 | Lim kinase 1 | LIMK-1 | LIM kinase | LIMK
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human LIMK1/2 around the phosphorylation site of Threonine 508/505.
Epitope
pThr508,pThr505
Specificity
Phospho-LIMK1/2 (Thr508/505) Antibody detects endogenous levels of LIMK1/2 only when phosphorylated at Threonine 508/505.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIMK1 / LIMK
P53667 NM_002314 NP_002305.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024