Target
Human LIG1 / DNA Ligase 1
Synonyms
LIG1 | DNA ligase I | DNA ligase 1 | Ligase I, DNA, ATP-dependent
Reactivity
Human, Monkey, Rat, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIG1 / DNA Ligase 1