IHC‑plus™ Monoclonal Mouse anti‑Human LIG1 / DNA Ligase 1 Antibody (clone 10G12, IHC, WB) LS‑B6111
IHC‑plus™ Monoclonal Mouse anti‑Human LIG1 / DNA Ligase 1 Antibody (clone 10G12, IHC, WB) LS‑B6111
Note: This antibody replaces LS-C133292
Antibody:
LIG1 / DNA Ligase 1 Mouse anti-Human Monoclonal (10G12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
LIG1 / DNA Ligase 1 Mouse anti-Human Monoclonal (10G12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
DNA Ligase 1 antibody LS-B6111 is an unconjugated mouse monoclonal antibody to human DNA Ligase 1 (LIG1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human LIG1 / DNA Ligase 1
Synonyms
LIG1 | DNA ligase I | DNA ligase 1 | Ligase I, DNA, ATP-dependent
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Immunogen
LIG1 (NP_000225, 810 aa ~ 920 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:1000)
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIG1 / DNA Ligase 1
Validation
Anti-LIG1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LIG1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human
Applications:
Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Monkey, Rat, Hamster
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/10/2025