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Catalog Number Size Price
LS-C352467-100 100 µl (1 mg/ml) $299 
LGALS3 / Galectin 3 Antibody - Immunohistochemical analysis of Galectin 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
LGALS3 / Galectin 3 Antibody - Immunofluorescent analysis of Galectin 3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
LGALS3 / Galectin 3 Antibody - Western blot analysis of Galectin 3 expression in HeLa (A); Jurkat (B); Raw264.7 (C); mouse liver (D); rat liver (E) whole cell lysates.
LGALS3 / Galectin 3 Antibody - Immunohistochemical analysis of Galectin 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
LGALS3 / Galectin 3 Antibody - Immunofluorescent analysis of Galectin 3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
LGALS3 / Galectin 3 Antibody - Western blot analysis of Galectin 3 expression in HeLa (A); Jurkat (B); Raw264.7 (C); mouse liver (D); rat liver (E) whole cell lysates.
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Polyclonal Rabbit anti‑Human LGALS3 / Galectin 3 Antibody (Internal, IHC, IF, WB) LS‑C352467

Polyclonal Rabbit anti‑Human LGALS3 / Galectin 3 Antibody (Internal, IHC, IF, WB) LS‑C352467

Antibody:
LGALS3 / Galectin 3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352467-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LGALS3 / Galectin 3 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Galectin 3 antibody LS-C352467 is an unconjugated rabbit polyclonal antibody to Galectin 3 (LGALS3) (Internal) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human LGALS3 / Galectin 3
Synonyms
LGALS3 | 35 kd lectin | 35 kDa lectin | CBP35 | GAL3 | Galactoside-binding protein | GALIG | IgE-binding protein | Laminin-binding protein | Gal-3 | GALBP | MAC-2 | Mac-2 antigen | L31 | MAC2 | CBP 35 | Galactose-specific lectin 3 | Galectin-3 | L-31 | Lectin L-29
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Galectin 3.
Epitope
Internal
Specificity
Recognizes endogenous levels of Galectin 3 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LGALS3 / Galectin 3
P17931 NM_002306 NP_002297.2

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Requested From: United States
Date Requested: 11/22/2024