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Catalog Number	Size	Price
LS-C407822-10	10 µg	$318
LS-C407822-100	100 µg	$470

LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody IHC-paraffin. IHC(P): Mouse Spleen Tissue.

LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody IHC-paraffin. IHC(P): Rat Spleen Tissue.

LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody Western blot. All lanes: Anti Lipocalin 2 at 0.5 ug/ml. Lane 1: Mouse Lung Tissue Lysate at 50 ug. Lane 2: Mouse Intestine Tissue Lysate at 50 ug. Predicted band size: 22 kD. Observed band size: 22 kD.

LCN2 / Lipocalin 2 / NGAL Antibody - Flow Cytometry analysis of A431 cells using anti-Lipocalin 2 antibody. Overlay histogram showing A431 cells stained with anti-Lipocalin 2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lipocalin 2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Rat LCN2 / Lipocalin 2 / NGAL Antibody (aa21‑198, IHC, WB) LS‑C407822

Name: Anti-LCN2 / Lipocalin 2 / NGAL Antibody | Rabbit anti-Rat | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407822-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

LCN2 / Lipocalin 2 / NGAL Rabbit anti-Rat Polyclonal (aa21-198) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Rat, Mouse

Format:

Unconjugated, Unmodified

$318

LS-C407822-10

Toll Free North America

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For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

LCN2 / Lipocalin 2 / NGAL Rabbit anti-Rat Polyclonal (aa21-198) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Rat, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

NGAL antibody LS-C407822 is an unconjugated rabbit polyclonal antibody to NGAL (LCN2 / Lipocalin 2) (aa21-198) from rat. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.

Target

Rat LCN2 / Lipocalin 2 / NGAL

Synonyms

See All LCN2 / Lipocalin 2 / NGAL Antibodies

Host

Rabbit

Reactivity

Rat, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198). Rat Lipocalin 2 shares 64.4 % and 81.1 % amino acid (aa) sequence identity with human and mouse Lipocalin 2, respectively.

Epitope

aa21-198

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About LCN2 / Lipocalin 2 / NGAL

Uniprot: P80188 NCBI: NM_005564 NP_005555.2