Mouse LAMC1 / Laminin Gamma 1

Rat

Mouse, Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

SPM193

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Murine EHS laminin preparation.

Mouse LAMC1 / Laminin Gamma 1

• IHC - Paraffin (0.5 - 1 µg/ml)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific mAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes. Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

PBS, BSA/sodium azide free

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P11047 NCBI: NM_002293 NP_002284.3