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Catalog Number	Size	Price
LS-C662118-10	10 µg	$318
LS-C662118-100	100 µg	$470

KPNA2 / Importin Alpha 1 Antibody - Western blot analysis of KPNA2 using anti-KPNA2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KPNA2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KPNA2 at approximately 58KD. The expected band size for KPNA2 is at 58KD.

Polyclonal Rabbit anti‑Human KPNA2 / Importin Alpha 1 Antibody (WB) LS‑C662118

Name: Anti-KPNA2 / Importin Alpha 1 Antibody | Rabbit anti-Human WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662118-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

KPNA2 / Importin Alpha 1 Rabbit anti-Human Polyclonal Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662118-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

KPNA2 / Importin Alpha 1 Rabbit anti-Human Polyclonal Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Importin Alpha 1 antibody LS-C662118 is an unconjugated rabbit polyclonal antibody to human Importin Alpha 1 (KPNA2). Validated for WB.

Target

Human KPNA2 / Importin Alpha 1

Synonyms

See All KPNA2 / Importin Alpha 1 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the N-Terminus of human KPNA2 (2-46 aa STNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQ), different from the related mouse sequence by three amino acids.

Specificity

Expressed ubiquitously.

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About KPNA2 / Importin Alpha 1

Uniprot: P52292 NCBI: NM_002266 NP_002257.1