Target
Human KLRB1 / CD161
Synonyms
KLRB1 | CD161 antigen | CLEC5B | CD161 | NKR-P1A | NKR | HNKR-P1A | NKR-P1 | NKRP1A
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
FITC,
Biotin,
PE.
Purification
Protein A purified
Modifications
Unmodified.
Also available
Preservative Free.
Immunogen
Activated human NK cells.
Specificity
Antibody B199.2 recognizes the dimeric 160 kd CD161 molecule. In MLR, blockade with antibody B199.2 caused a slight but sigfnificant reduction in the percentage of IFN gamma-expressing NK cells challenged with M. tuberculosis
Usage
Five x 105 ficoll prepared human peripheral blood mononuclear cells per tube were pre incubated 5 minutes with 250 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD161 antibody at 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 22% sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee