Target
Human KLK15 / Kallikrein 15
Synonyms
KLK15 | ACO protease | ACO | HSRNASPH | Kallikrein 15 | Prostinogen | Kallikrein-15
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
NS0-derived recombinant human KLK15 isoform 1 (aa17-256).
Specificity
Selected for its ability to recognize human KLK15 in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1% cross-reactivity with rhKLK2, rhKLK8, and rhKLK11.
Applications
- Western blot
- Immunoprecipitation
- ELISA
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human KLK15. The detection limit for rhKLK15 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK15 from conditioned media of transfected NS0 cells. The recovered rhKLK15 can be detected by Western blot analysis using a monoclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human KLK15. The detection limit for rhKLK15 is approximately 0.5 ng/well.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute with 500ul sterile PBS
Storage
Store at -20°C lyophilized. Once reconstituted: Store at -20°C. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KLK15 / Kallikrein 15