Target
Human KDR / VEGFR2 / FLK1
Synonyms
KDR | CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
Reactivity
Human, Mouse, Drosophila melanogaster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Immunogen
Synthetic peptide corresponding to residues surrounding Tyr1175 of human VEGF receptor 2 (KLH coupled).
Specificity
Recognizes endogenous levels of human VEGFR-2 only when phosphorylated at tyrosine 1175 at ~230kD. Does not crossreact with other phosphorylated receptor tyrosine kinases. May cross react with VEGFR1.
Applications
- ICC
- Immunofluorescence (1:200)
- Western blot (1:1000)
- ELISA
Usage
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Western Blot: 1:1000. Immunohistochemistry (paraffin): 1:300. Immunofluorescence (IC): 1:100.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KDR / VEGFR2 / FLK1