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Catalog Number Size Price
LS-C407689-10 10 µg $318 
LS-C407689-100 100 µg $470 
Junctin / ASPH Antibody - IHC analysis of ASPH using anti-ASPH antibody. ASPH was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ASPH Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Junctin / ASPH Antibody - ASPH antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
Junctin / ASPH Antibody - ASPH antibody Western blot. All lanes: Anti ASPH at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: HEPG2 Whole Cell Lysate at 40 ug. Lane 5: HEPA Whole Cell Lysate at 40 ug. Predicted band size: 86 kD. Observed band size: 100 kD.
Junctin / ASPH Antibody - IHC analysis of ASPH using anti-ASPH antibody. ASPH was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ASPH Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Junctin / ASPH Antibody - ASPH antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
Junctin / ASPH Antibody - ASPH antibody Western blot. All lanes: Anti ASPH at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: HEPG2 Whole Cell Lysate at 40 ug. Lane 5: HEPA Whole Cell Lysate at 40 ug. Predicted band size: 86 kD. Observed band size: 100 kD.
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Polyclonal Rabbit anti‑Human Junctin / ASPH Antibody (aa726‑758, IHC, WB) LS‑C407689

Polyclonal Rabbit anti‑Human Junctin / ASPH Antibody (aa726‑758, IHC, WB) LS‑C407689

Antibody:
Junctin / ASPH Rabbit anti-Human Polyclonal (aa726-758) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Guinea pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407689-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Junctin / ASPH Rabbit anti-Human Polyclonal (aa726-758) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Guinea pig
Format:
Unconjugated, Unmodified

Specifications

Description
ASPH antibody LS-C407689 is an unconjugated rabbit polyclonal antibody to ASPH (Junctin) (aa726-758) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human Junctin / ASPH
Synonyms
ASPH | A beta H-J-J | AAH | ASP beta-hydroxylase | BAH | Aspartate beta-hydroxylase | CASQ2BP1 | Cardiac junctin | HAAH | Humbug | JCTN | Junctate | Junctin
Host
Rabbit
Reactivity
Human, Mouse, Rat, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human ASPH (726-758 aa EVWQDASSFRLIFIVDVWHPELTPQQRRSLPAI), identical to the related mouse sequence.
Epitope
aa726-758
Specificity
Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level). .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Junctin / ASPH
Q12797 NM_004318 NP_004309.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/22/2024