Target
Human JAG1 / Jagged 1
Synonyms
JAG1 | AGS | AHD | Alagille syndrome | CD339 antigen | HJ1 | Jagged1 | JAGL1 | Jagged | Jagged 1 | Protein jagged-1 | AWS | CD339 | Soluble protein jagged
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
This protein A purified Jagged 1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the N-Terminus of human Jagged-1 protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.
Applications
- IHC (1:100 - 1:500)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:500 - 1:2000)
- ELISA (1:20000 - 1:100000)
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Usage
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kD in size corresponding to Jagged-1 in mouse liver whole cell lysates, and a 75 kD band in human brain and kidney lysates.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee