Human ITGB4 / Integrin Beta 4

Rat

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

439-9B

Unconjugated. Also available conjugated with Biotin.

Affinity purified

Unmodified

Human ITGB4

• IHC
• IHC - Paraffin (20 µg/ml)
• IHC - Frozen
• Western blot
• Flow Cytometry

This antibody is for use in Western Blot only under non-reducing conditions. Immunohistochemistry: LS-B3778 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3778 was determined to be 20 ug/ml. Tested by flow cytometric analysis of A549 cell line. Antibody can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.. Sourced from tissue culture supernatant

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16144 NCBI: NM_000213 NP_000204.3