Human ITGB4 / Integrin Beta 4

Rabbit

Human, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human b4 integrin subunit (1). The sequence used is NH2- (K)GTLSTHMDQQFFQT-amide. The lysine (K) is not in the b4 sequence and was added to conjugate the peptide to the protein carrier. Percent identity by BLAST analysis: Human (100%); Mouse, Rat, Hamster, Bovine (93%); Gorilla, Gibbon, Horse (86%).

aa1809-1822

Recognizes human b4 integrin as determined by immunoprecipitation. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. No cross-reactivity to other integrin b subunits. Species cross-reactivity: rat and mouse.

• IHC
• IHC - Frozen
• Immunoprecipitation

Suitable for use in Immunoprecipitation. Immunoprecipitation: Labeled cell extract 5 ul of serum is sufficient to precipitate a6b4 from 1-2 x 1E6 cells. In some cases immunoprecipitation from 35S methionine labeled cells originates two bands of 95 and 55kD of unknown origin in addition to the 200kD and 140kD bands corresponding to b4 and a6.

0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16144 NCBI: NM_000213 NP_000204.3