Monoclonal Mouse anti‑Human ITGB2 / CD18 Antibody (clone MEM‑148, Preservative Free, Activation Epitope, WB) LS‑C44253
Monoclonal Mouse anti‑Human ITGB2 / CD18 Antibody (clone MEM‑148, Preservative Free, Activation Epitope, WB) LS‑C44253
Antibody:
ITGB2 / CD18 Mouse anti-Human Monoclonal (Activation Epitope) (Preservative Free) (MEM-148) Antibody
Format:
Unconjugated, Preservative Free
Overview
Antibody:
ITGB2 / CD18 Mouse anti-Human Monoclonal (Activation Epitope) (Preservative Free) (MEM-148) Antibody
Format:
Unconjugated, Preservative Free
Specifications
Description
CD18 antibody LS-C44253 is an unconjugated mouse monoclonal antibody to human CD18 (ITGB2) (Activation Epitope). Validated for Flow, IP and WB.
Target
Human ITGB2 / CD18
Synonyms
ITGB2 | CD18 antigen | CD18 | Integrin beta chain, beta 2 | LCAMB | MAC-1 | MFI7 | MF17 | Integrin beta-2 | LAD | LFA-1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Preservative Free.
Also available
Unmodified.
Immunogen
Peripheral blood mononuclear cells. (ACTIVATION EPITOPE).
Epitope
Activation Epitope
Specificity
Recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH.
Applications
- Western blot
- Immunoprecipitation
- Flow Cytometry (1:20 - 1:200)
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Storage
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Species:
Human, Mouse, Rat
Applications:
Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Species:
Human
Applications:
IHC, IHC - Frozen, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Reactivity:
Human
Range:
0.313-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/5/2025