Rat ITGAM / CD11b

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

OX-42

Unconjugated

Protein G purified

Low Endotoxin

Rat peritoneal macrophages

Recognizes the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain. Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes(1).

• IHC - Frozen (1:50 - 1:100)
• Immunofluorescence
• Immunoprecipitation
• Flow Cytometry (1:50 - 1:100)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P11215 NCBI: NM_000632 NP_000623.2