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Catalog Number Size Price
LS-C407860-10 10 µg $318 
LS-C407860-100 100 µg $470 
ITGA4 / VLA-4 / CD49d Antibody - IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody. Integrin alpha 4 was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Integrin alpha 4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ITGA4 / VLA-4 / CD49d Antibody - Integrin alpha 4 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
ITGA4 / VLA-4 / CD49d Antibody - Integrin alpha 4 antibody Western blot. All lanes: Anti Integrin alpha 4 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Lane 4: 22RV1 Whole Cell Lysate at 40 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Lane 6: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 115 kD. Observed band size: 220 kD.
ITGA4 / VLA-4 / CD49d Antibody - Flow Cytometry analysis of THP-1 cells using anti-Integrin alpha 4 antibody. Overlay histogram showing THP-1 cells stained with anti-Integrin alpha 4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ITGA4 / VLA-4 / CD49d Antibody - IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody. Integrin alpha 4 was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Integrin alpha 4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ITGA4 / VLA-4 / CD49d Antibody - Integrin alpha 4 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
ITGA4 / VLA-4 / CD49d Antibody - Integrin alpha 4 antibody Western blot. All lanes: Anti Integrin alpha 4 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: JURKAT Whole Cell Lysate at 40 ug. Lane 4: 22RV1 Whole Cell Lysate at 40 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Lane 6: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 115 kD. Observed band size: 220 kD.
ITGA4 / VLA-4 / CD49d Antibody - Flow Cytometry analysis of THP-1 cells using anti-Integrin alpha 4 antibody. Overlay histogram showing THP-1 cells stained with anti-Integrin alpha 4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human ITGA4 / VLA‑4 / CD49d Antibody (aa999‑1028, IHC, WB) LS‑C407860

Polyclonal Rabbit anti‑Human ITGA4 / VLA‑4 / CD49d Antibody (aa999‑1028, IHC, WB) LS‑C407860

Antibody:
ITGA4 / VLA-4 / CD49d Rabbit anti-Human Polyclonal (aa999-1028) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407860-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ITGA4 / VLA-4 / CD49d Rabbit anti-Human Polyclonal (aa999-1028) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD49d antibody LS-C407860 is an unconjugated rabbit polyclonal antibody to CD49d (ITGA4 / VLA-4) (aa999-1028) from human. It is reactive with human and rat. Validated for IHC and WB.
Target
Human ITGA4 / VLA-4 / CD49d
Synonyms
ITGA4 | 269C wild type | CD49D | IA4 | Integrin alpha-4 | Integrin alpha 4 | Integrin alpha-4 subunit | Integrin alpha-IV | VLA-4 | VLA-4 subunit alpha | CD49d antigen
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Integrin alpha 4 (999-1028 aa MWKAGFFKRQYKSILQEENRRDSWSYINS K), different from the related mouse sequence by one amino acid.
Epitope
aa999-1028
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGA4 / VLA-4 / CD49d
P13612 NM_000885 NP_000876.3

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Requested From: United States
Date Requested: 4/25/2024