Human ITGA4 / VLA-4 / CD49d

Rabbit

Human, Mouse, Rat, Hamster, Chicken (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

Synthetic peptide derived from the C-Terminus sequence of the human alpha 4 integrin subunit. Cellular Localization: Cytoplasmic; Intracellular.

C-Terminus

Recognizes human Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. Recognizes an epitope on the cytoplasmic domain of the protein. Epitope is intracellular. Species cross-reactivity: rat, chicken, hamster and mouse.

• IHC (1:250)
• Western blot (1:1000)
• Immunoprecipitation
• ELISA (1:1000)
• Radioimmunoassay (1:500)

Suitable for use in ELISA, Radioimmunoassay, Western Blot, Immunohistochemistry and Immunoprecipitation. ELISA/RIA: 1:500. Western Blot (non-reduced): 1:500. Blocking should be with 1-3% BSA. Recognizes a 140kD band under non-reducing conditions. Immunohistochemistry: 1:250. Immunoprecipitation: 5 ul to precipitate alpha4 from 5x1E6 cells.

0.05% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13612 NCBI: NM_000885 NP_000876.3