Target
Human ITGA3 / CD49c
Synonyms
ITGA3 | Alpha3 integrin | CD49c antigen | CD49C | GAPB3 | Integrin alpha-3 | FRP-2 | VCA-2 | VLA-3 subunit alpha | VL3A | VLA3a | Galactoprotein B3 | GAP-B3 | ILNEB | Integrin alpha3 | MSK18
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Human squamous cell carcinoma cells (SCC9).
Specificity
Recognized human a3 integrin (CD49c).
Applications
- IHC
- IHC - Frozen
- Immunoprecipitation
- Flow Cytometry
- Functional Assay
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Usage
Suitable for use in Inhibition of Cell Attachment, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Immunoprecipitation: Precipitates PAGE bands of 130kD (reduced) or 116kD and 135kD (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.
Presentation
PBS, pH 7.6, 0.1% Sodium Azide
Storage
Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee