Human ITGA3 / CD49c

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Purified

Unmodified

Human renal carcinoma cell line (KJ29).

Recognizes an epitope related to the VLA-3 complex (a3/b1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of 35S-methionine labeled KJ29 cells. Insolubilized antibody is capable of retaining both chains in a wide range of pH values (2 to 10.5) with no preferential loss of the a3/b1 molecule. Recognizes two chains with apparent MW of 110 and 150kD. In the presence of a reducing agent (2%B-ME), a single MW of 130kD is detected.

• ICC
• Immunoprecipitation
• Flow Cytometry
• ELISA
• Radioimmunoassay

• IHC - Paraffin

Suitable for use in Immunohistochemistry, ELISA, RIA, Immunoprecipitation, Immunocytochemistry and FACS. Immunohistochemistry: Acetone fixed, paraffin embedded sections. Immunoprecipitation: Under non-reducing conditions, two chains with apparent molecular weight of 110 and 150kD. In the presence of a reducing agent (2% B-ME), a single molecular species of 130kD is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight. Positive control: Cell Line: KJ29 renal carcinoma adherent cells.

PBS, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P26006 NCBI: NM_002204 NP_002195.1