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Catalog Number	Size	Price
LS-C757844-200	200 µl	$393

IRAK1 / IRAK Antibody - Phospho-IRAK1(S376) Antibody staining IRAK1 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

IRAK1 / IRAK Antibody - Phospho-IRAK1(S376) Antibody staining IRAK1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

IRAK1 / IRAK Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling IRAK1 with Phospho-IRAK1(S376) Antibody at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nuclear speckles staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

IRAK1 / IRAK Antibody - Western blot analysis of lysates from Hela cell line, untreated or treated with IL-1 beta (20ng/ml) + Calyculin A (100nM), using (upper) or Tubulin (lower).

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Polyclonal Rabbit anti‑Human IRAK1 / IRAK Antibody (phospho‑Ser376, IHC, IF, WB) LS‑C757844

Name: Anti-IRAK1 / IRAK Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C757844-200
Price: 393.0000 USD
Availability: InStock

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Antibody:

IRAK1 / IRAK Rabbit anti-Human Polyclonal (pSer376) Antibody

Application:

IHC-P, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C757844-200

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

IRAK1 / IRAK Rabbit anti-Human Polyclonal (pSer376) Antibody

Application:

IHC-P, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

IRAK antibody LS-C757844 is an unconjugated rabbit polyclonal antibody to human IRAK (IRAK1) (pSer376). Validated for ELISA, IF, IHC and WB.

Target

Human IRAK1 / IRAK

Synonyms

See All IRAK1 / IRAK Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Ig Polyclonal

Conjugations

Unconjugated

Purification

Protein A and Peptide Affinity Chromatography

Modifications

Unmodified

Immunogen

This Phospho-IRAK1(S376) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 348-381 amino acids from human IRAK1.

Epitope

pSer376

Applications

IHC - Paraffin (1:25)
Immunofluorescence (1:25)
Western blot (1:1000)
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Western Blot Predicted Molecular Weight: 76.5 kDa

Presentation

PBS, 0.09% Sodium Azide

Storage

Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IRAK1 / IRAK