Target
Human ILT3 / LILRB4
Synonyms
LILRB4 | CD85K | CD85k antigen | ILT-3 | ILT3 | LIR-5 | LIR5 | HM18
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhILT3 extracellular domain.
Epitope
Extracellular Domain
Specificity
Recognizes human ILT3. In Western Blots, this antibody does not cross-react with rhILT2, 4, 5, 6, rhLIR6, or rhLIR8.
Usage
Western Blot, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human ILT3. Using a colorimetric detection system, the detection limit for rhILT3 is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human ILT3. The detection limit for rhILT3 is approximately 1 ng/well.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Prior to reconstitution store at -20°C. After reconstitution store at -20°C. Store undiluted. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee