Human IL4R / CD124

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Monoclonal

R401

Unconjugated

Protein A purified

Preservative Free

Recombinant Human IL-4R / CD124 Protein

aa1-232

Human IL4R. No cross-reactivity with Mouse IL4R and Rat IL4R in ELISA assay

• ELISA (1.2 µg/ml)
• Blocking (2 µg/ml)
• Neutralization (0.2 ng/ml)

Block: In a functional ELISA, 1. 2 µg/ml of the Rabbit Anti-Human IL4R Monoclonal antibody will block 50% of the binding of 2 µg/ml of Human IL4 to immobilized Recombinant Human IL4R ) coated at 0.5 µg/ml (100 µL/well). At 5 ug/ml, this antibody will block >90% of the binding. Neutralization: The neutralization activity of IL4R Neutralizing Antibody is Measured by its ability to neutralize IL4 induced proliferation in the human erythroleukemia cell line (TF-1). The Neutralization titer (IC50) is typically 1-4 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL4. Please Note: Optimal concentrations/dilutions should be determined by the end user.

0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24394 NCBI: NM_000418 NP_000409.1