Target
Mouse IL4
Synonyms
IL4 | B cell growth factor 1 | B-cell stimulatory factor 1 | B_cell stimulatory factor 1 | BCGF1 | BSF-1 | Binetrakin | BCGF-1 | Interleukin 4 | Interleukin-4 | Pitrakinra | BSF1 | IL-4
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated
Immunogen
E. coli-expressed recombinant mouse IL-4.
Specificity
Recognizes mouse Interleukin-4 (IL-4).
Applications
- Immunofluorescence
- Western blot
- Flow Cytometry
- ELISA
Usage
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-4 under reducing conditions.
Presentation
100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee