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Recombinant Monoclonal Rabbit anti‑Human IL2RA / CD25 Antibody (clone Basiliximab, IL‑2R alpha (CD25), IHC, IF, WB) LS‑C777504
Recombinant Monoclonal Rabbit anti‑Human IL2RA / CD25 Antibody (clone Basiliximab, IL‑2R alpha (CD25), IHC, IF, WB) LS‑C777504
Antibody:
IL2RA / CD25 Rabbit anti-Human Recombinant Monoclonal (IL-2R alpha (CD25)) (Basiliximab) Antibody
Application:
IHC, IF, WB, Flo, Block
Reactivity:
Human, Monkey-Cynomolgus, Monkey-Rhesus
Format:
Unconjugated, Unmodified
Overview
Antibody:
IL2RA / CD25 Rabbit anti-Human Recombinant Monoclonal (IL-2R alpha (CD25)) (Basiliximab) Antibody
Application:
IHC, IF, WB, Flo, Block
Reactivity:
Human, Monkey-Cynomolgus, Monkey-Rhesus
Format:
Unconjugated, Unmodified
Specifications
Description
This IL2RA / CD25 antibody is an unconjugated Rabbit/Human chimeric recombinant monoclonal antibody. Validated for Block, Flo, IF, IHC, and WB.
Target
Human IL2RA / CD25
Synonyms
IL2RA | CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
Reactivity
Human, Monkey-Cynomolgus, Monkey-Rhesus
(tested or 100% immunogen sequence identity)
Clonality
IgG,k
Recombinant Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
Epitope
IL-2R alpha (CD25)
Specificity
Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ( alpha beta gamma c) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Applications
- IHC (5 µg/ml)
- Immunofluorescence (5 µg/ml)
- Western blot
- Flow Cytometry
- Blocking
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
PBS, 0.02% ProClin™ 300.
Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, Flow Cytometry
Species:
Human
Applications:
Western blot
Species:
Human
Applications:
Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024