Human IL2RA / CD25

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

Unconjugated

Protein G purified

Unmodified

Insect cell derived recombinant Human sIL-2Ralpha.

Human IL-2Ralpha

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen (5 µg/ml)
• Western blot (0.2 - 0.4 µg/ml)
• ELISA (4 - 8 µg/ml)

Immunohistochemistry: LS-B7396 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2-4 ng/lane, under non-reducing conditions.

Lyophilized from Sterile Solution PBS

Centrifuge vial prior to opening. Reconstitiute in sterile water to 1.0 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01589 NCBI: NM_000417 NP_000408.1