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Catalog Number	Size	Price
LS-C662082-10	10 µg	$318
LS-C662082-100	100 µg	$470

IL17RA Antibody - Western blot analysis of IL17RA using anti-IL17RA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate,Lane 3: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17RA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17RA at approximately 160KD. The expected band size for IL17RA is at 96KD.

Polyclonal Rabbit anti‑Human IL17RA Antibody (WB) LS‑C662082

Name: Anti-IL17RA Antibody | Rabbit anti-Human Polyclonal ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662082-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

IL17RA Rabbit anti-Human Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662082-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IL17RA Rabbit anti-Human Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

IL17RA antibody LS-C662082 is an unconjugated rabbit polyclonal antibody to human IL17RA. Validated for ELISA and WB.

Target

Human IL17RA

Synonyms

See All IL17RA Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human IL17RA recombinant protein (Position: K53-Q284). Human IL17RA shares 74.1% amino acid (aa) sequence identity with mouse IL17RA.

Specificity

Widely expressed.

Applications

Western blot
ELISA

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IL17RA

Uniprot: Q96F46 NCBI: NM_014339 NP_055154.3