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| Catalog Number | Size | Price |
|---|---|---|
| LS-A9075-50 | 50 µg (1 mg/ml) | $440 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human WNT10A Antibody (Internal, IHC) LS‑A9075
IHC‑plus™ Polyclonal Rabbit anti‑Human WNT10A Antibody (Internal, IHC) LS‑A9075
Note: This antibody replaces LS-C90726, LS-C42080
Antibody:
WNT10A Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bat, Bovine, Dog, Rabbit
Format:
Unconjugated, Unmodified
Overview
Antibody:
WNT10A Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bat, Bovine, Dog, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
WNT10A antibody LS-A9075 is an unconjugated rabbit polyclonal antibody to WNT10A (Internal) from human. It is reactive with human, bat, bovine and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human WNT10A
Synonyms
WNT10A | SSPS | STHAG4 | OODD | Protein Wnt-10a
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Dog, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Rat, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from internal region of human WNT10A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bovine, Bat, Elephant, Panda, Rabbit (100%); Rat, Hamster, Opossum (94%).
Epitope
Internal
Specificity
Human WNT10A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PTPRD (44%).
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About WNT10A
Validation
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Stomach: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Stomach: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-WNT10A antibody IHC of human pancreatic islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT10A antibody IHC of human pancreatic islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Human Melanoma (Lymph Node): Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Melanoma (Lymph Node): Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ WNT10A Antibody (Internal) for IHC LS-A9075 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Mouse, Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Bovine, Dog
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
