Bovine Ubiquitin

Mouse

Bovine, Human, Mouse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Purified bovine ubiquitin conjugated to carrier protein.

Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• Immunofluorescence
• Western blot
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B3328 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3328 was determined to be 10 ug/ml. Western Blot: 1-3 ug/ml; Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl, pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.