Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B14320-50 50 µl (1 mg/ml) $460 
TP53 / p53 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TP53 / p53 Antibody - Immunofluorescent analysis of p53 (pS20) staining in HCT116 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TP53 / p53 Antibody - Western blot analysis of p53 (pS20) expression in HCT116 (A); COS7 (B) whole cell lysates.
TP53 / p53 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TP53 / p53 Antibody - Immunofluorescent analysis of p53 (pS20) staining in HCT116 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TP53 / p53 Antibody - Western blot analysis of p53 (pS20) expression in HCT116 (A); COS7 (B) whole cell lysates.
1 of 3
2 of 3
3 of 3

IHC‑plus™ Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (phospho‑Ser20, IHC, IF, WB) LS‑B14320

IHC‑plus™ Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (phospho‑Ser20, IHC, IF, WB) LS‑B14320

Note: This antibody replaces LS-C368613
Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (pSer20) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14320-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (pSer20) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-B14320 is an unconjugated rabbit polyclonal antibody to p53 (TP53) (pSer20) from human. It is reactive with human, monkey, pig and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Rabbit
Reactivity
Human, Monkey, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human p53.
Epitope
pSer20
Specificity
Recognizes endogenous levels of p53 (pS20) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

Validation

TP53 / p53 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ TP53 / p53 Antibody (phospho-Ser20) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B14320 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/25/2024