Human TP53 / p53

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

DO-11

Unconjugated

Purified IgG

Unmodified

Recombinant human p53 (AA 181-190).

aa181-190

Recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kD cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells. DO-11 binds to an epitope within the central region of p53 between amino acids defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promoter usage, the epitope recognized by clone DO-11 is present in all isoforms. Recognizes mutant forms of p53 and has been used successfully for detection of p53 by western blotting.

• IHC - Paraffin (1:1000)
• IHC - Frozen
• Western blot (1:1000)
• Immunoprecipitation

Immunohistology: This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.

PBS, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04637 NCBI: NM_000546 NP_000537.3