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Catalog Number Size Price
LS-B430-50 50 µg (1 mg/ml) $515 
TLR8 Antibody - Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR8 Antibody - Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.
TLR8 Antibody - Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR8 Antibody - Western blot of Rabbit Anti-TLR8 Antibody. Lane 1: Daudi whole cell lysate primary antibody diluted to 1:2000. Lane 2: Daudi whole cell lysate primary antibody diluted to 1:1000. Lane 3: Daudi whole cell lysate primary antibody diluted to 1:500. Load: 10 ug per lane. Primary antibody: TLR 8 antibody overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115 kDa, 115 kDa for TLR-8. Other band(s): none.
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IHC‑plus™ Polyclonal Rabbit anti‑Human TLR8 Antibody (Internal, IHC, WB) LS‑B430

IHC‑plus™ Polyclonal Rabbit anti‑Human TLR8 Antibody (Internal, IHC, WB) LS‑B430

Note: This antibody replaces LS-C19085
Antibody:
TLR8 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B430-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TLR8 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
TLR8 antibody LS-B430 is an unconjugated rabbit polyclonal antibody to TLR8 (Internal) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TLR8
Synonyms
TLR8 | CD288 | Toll-like receptor 8 | CD288 antigen
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human TLR8.
Epitope
Internal
Specificity
An internal region of human TLR8 protein.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B430 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B430 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR8
Q9NR97 NM_138636 NP_619542.1

Validation

TLR8 Antibody - Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR8 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ TLR8 Antibody (Internal) for IHC, WB/Western LS-B430 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/21/2024