Human TGM2 / Transglutaminase 2

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

Recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.

Recognizes human Transglutaminase II at ~77-85kD.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (5 - 10 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B2235 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2235 was determined to be 5 ug/ml. Immunoprecipitation (Native verified): 10 ug/mg protein lysate. Use Protein A. Western Blot: 5-10 ug/ml for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma.

PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA

Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P21980 NCBI: NM_004613 NP_004604.2