Human TGM2 / Transglutaminase 2

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Azide-free

Recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.

Recognizes Transglutaminase II. Species cross-reactivity: Human, mouse, rat.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (5 - 10 µg/ml)
• Immunoprecipitation (10 µg/tst)

Immunohistochemistry: LS-B2681 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2681 was determined to be 5 ug/ml. Immunoprecipitation (Native verified): 10 ug/mg protein lysate. Use Protein A. Western Blot: 5-10 ug/ml for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, pH 7.4

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P21980 NCBI: NM_004613 NP_004604.2