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Catalog Number	Size	Price
LS-B11086-200	200 µl (0.5 mg/ml)	$460

TERT / Telomerase Antibody - Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

TERT / Telomerase Antibody - Fluorescent confocal image of HeLa cell stained with TERT Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

TERT / Telomerase Antibody - Fluorescent confocal image of SY5Y cells stained with TERT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated TERT primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.

TERT / Telomerase Antibody - Western blot analysis of Anti-TERT antibody (LS-B11086, 1:2000 dilution; 20 µg lysates/proteins per lane). Lane 1: HeLa whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: MOLT-4 whole cell lysate. Lane 5: PC-3 whole cell lysate. Lane 6: 293 whole cell lysate. Lane 7: COLO 205 whole cell lysate. Antibody produced bands at ~127 kDa.

TERT / Telomerase Antibody - TERT Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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IHC‑plus™ Polyclonal Rabbit anti‑Human TERT / Telomerase Antibody (aa627‑656, IHC, IF, WB) LS‑B11086

Name: IHC-plus™ TERT / Telomerase Polyclonal Antibody Rabbit | LSBio
Brand: Lifespan BioSciences
SKU: LS-B11086-200
Price: 460.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

IHC Validated

(5.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C98109

Antibody:

TERT / Telomerase Rabbit anti-Human Polyclonal (aa627-656) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B11086-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TERT / Telomerase Rabbit anti-Human Polyclonal (aa627-656) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Telomerase antibody LS-B11086 is an unconjugated rabbit polyclonal antibody to human Telomerase (TERT) (aa627-656). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

(1) Publication(s)

Target

Human TERT / Telomerase

Synonyms

See All TERT / Telomerase Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa627-656

Specificity

This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-656 amino acids from the Central region of human TERT.

Applications

IHC
IHC - Paraffin (1:100)
Immunofluorescence (1:10 - 1:50)
Western blot (1:2000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TERT / Telomerase

Uniprot: O14746 NCBI: AY007685 AAG23289.1

Validation

Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

See More About...

About LSBio Validation process

LSBio Ratings

IHC-plus™ TERT / Telomerase Antibody (aa627-656) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B11086 has an LSBio Rating of

(5.0) Rating

Publications (4)

Laboratory Validation Score (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

MicroRNA-22 enhances radiosensitivity in cervical cancer cell lines via direct inhibition of c-Myc binding protein, and the subsequent reduction in hTERT expression. Mayumi Nakamura, Masami Hayashi, Hiromi Konishi, Misa Nunode, Keisuke Ashihara, Hiroshi Sasaki, Yoshito Terai, Masahide Ohmichi. Oncology letters. 2020 Mar;19:2213-2222.

Full Text Article PubMed: 32194719 PMC: PMC7038919

Submit a Publication for LS-B11086