Bovine SOD1 / Cu-Zn SOD

Mouse

Bovine (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

2F5

Unconjugated

Protein G purified

Unmodified

Superoxide dismutase enzyme prepared from bovine erythrocytes

Recognizes copper/zinc superoxide dismutase (SOD). Superoxide dismutases are ubiquitous metalloproteins that destroy oxygen-mediated free radicals, that are normally produced within the cells, which are toxic to biological systems. There are three forms of superoxide dismutase, including the Fe, mitochondrial (Mn) and the copper/zinc binding (Cu/Zn) form. The Cu/Zn form of SOD is utilized by most eukaryotic organisms. SOD Cu/Zn prevents oxygen-mediated free radical damage by catalyzing the dismutation of the toxic superoxide radical to molecular oxygen and hydrogen peroxide. Does not cross react with immunoglobulins but does react weakly with Mn SOD.

• IHC
• IHC - Paraffin (15 µg/ml)
• IHC - Frozen
• ELISA

Immunohistochemistry: LS-B3283 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3283 was determined to be 15 ug/ml. Histology Positive Control Tissue: Human spleen and lymph node.

PBS, 0.1% Sodium Azide, 0.1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00441 NCBI: NM_000454 NP_000445.1