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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2487-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human SKP2 Antibody (IHC, WB) LS‑B2487
IHC‑plus™ Monoclonal Mouse anti‑Human SKP2 Antibody (IHC, WB) LS‑B2487
Note: This antibody replaces LS-C9423
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
SKP2 antibody LS-B2487 is an unconjugated mouse monoclonal antibody to human SKP2. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human SKP2
Synonyms
SKP2 | F-box/LRR-repeat protein 1 | FBL1 | FLB1 | F-box protein Skp2 | FBXL1 | p45 | p45skp2
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant, full-length human p45SKP2 protein.
Specificity
Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2487 was determined to be 5 ug/ml. Positive control: HeLa, Jurkat and 293T cell lines.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SKP2
Validation
Anti-SKP2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SKP2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ SKP2 Antibody for IHC, WB/Western, IP, ELISA LS-B2487 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Damage-induced BRCA1 phosphorylation by Chk2 contributes to the timing of end resection. Parameswaran B, Chiang HC, Lu Y, Coates J, Deng CX, Baer R, Lin HK, Li R, Paull TT, Hu Y. Cell cycle (Georgetown, Tex.). 2015 14:437-48.
Phosphorylation of BRCA1 by ATM upon double-strand breaks impacts ATM function in end-resection: A potential feedback loop. Leilei Qi, Reka Chakravarthy, Monica M Li, Chu-Xia Deng, Rong Li, Yanfen Hu. iScience. 2022 September;25:104944.
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Source:
E. coli
Tag:
6His-SUMO, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
